RNA N6-methyladenosine (m⁶A) has been reported to control diverse genetic information and metabolic switches including RNA decay, translation efficiency, nuclear retention, and splicing. Transcriptome-wide profile of m6A has been revealed in maize. In our SMEP, users could easily enquiry and predict the potential m⁶A site on targeted DNA regions.

Example:

>test

ATTGACGGGTTAATGTGAGCTTATCCATGCGGTTAGGCACTCTTCAAATAGGAATCCATTTTCTAACTGGCTTTTTATCAGTAACCTTCTATGGCTAAATATTTGTATGTTGACTTTCCTTCTATCCATCCATCCACAGGAGCGAAGGTAAAGGTTGAGTGCAGATCGAAATCCACTGGCGCCAAGACATGCAGCTTCGAGGGCCAGACTGACCACACTGGCACCTACAACATCCCTGTCAACGATGAGCATGAGCACGAGCTCTGCGAGTCTGTCCTTGTGAGCAGCCCGGACGCAAAGTGTGGCAAGATTGTTGCTGGACGGGAGAGGGCTCCTGTCTTCCTCACCAACAACAATGGTGTGACATCTAATGTTCGGTTGGCGAACGCTCTGGGCTTCCAGAAGGATGCTCCTCTTGCTGCGTGCGCACAAATCCTCAAGATGTACGAGGAAGTGGACGACCGCGTTTGAAGGATGTGAGTTATGTATCAATATTCGTACGTTGTTGAAATACTCTAAGAAGACTTCTATATACCTTTTACTGAGAGCAAAACTATCAGCATCAGATGTTGCGTGCTTGATAAGTTGTGATATAGCTTA

Note:

As RNA m⁶A was experimentally detected by m⁶A-IP-seq with specific anti-m⁶A antibody, this experiment yields peak sequences and cannot recognize single nt sites on mRNA, we have trained this content using peak sequences. Thus, this example for m⁶A prediction by SMEP model indicates peak sequences.

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