Reverse genetics is a powerful tool for funtional genomics study. Chemical mutagenesis efficiently generates phenotypic variations in otherwise homogeneous genetic backgrounds, enabling linking biological function with a gene of interest. Using ethyl methanesulfonate (EMS), we mutagenized the fresh pollen from maize B73 inbred and obtained a large number of mutants. Exome capture- Based SNP Discovery ( EBSD) was used to identify all mutated loci. A website, called Maize EMS induced Mutant Database ( MEMD), is constructed to allow scientists to search for and order the mutant seeds of interest for non-profit research. We will keep updating all the information related to the mutants.
Link to MEMD
|